Supplementary Data from Combined Inhibition of p38MAPK and PIKfyve Synergistically Disrupts Autophagy to Selectively Target Cancer Cells

Abstract

<p>Supplementary Figure S1: Normal cells have consistently higher resistance to PIKfyve and p38MAPK inhibition than cancer cells. Supplementary Figure S2: JNK and p44/p42 MAP kinases protein and phosphorylation levels do not correlate with sensitivity to PIKfyve inhibition. Supplementary Figure S3: Manipulation of p38MAPK protein levels in resistant and sensitive cells changes their sensitivity to PIKfyve inhibition. Supplementary Figure S4: Synergistic cytotoxic effects of combined PIKfyve and p38MAPK inhibition. Supplementary Figure S5: Total cell count and SEMs in support of the data presented in Fig.3. Supplementary Figure S6: Inhibition of PIKfyve and/or p38MAPK kinases does not trigger apoptosis or activate the p53 stress response. Supplementary Figure S7: Combined PIKfyve and p38MAPK inhibition blocks autophagy in all cells. Supplementary Figure S8: WX8 is a specific inhibitor of PIKfyve.</p>