Supplemental Tables S1-3, Supplemental Figures S1-8 from Characterization and Evidence of the miR-888 Cluster as a Novel Cancer Network in Prostate

Abstract

<p>This supplemental data file consists of 3 supplemental tables and 8 supplemental figures. Suppl. Tables 1-2 lists specific miRNA mimics, inhibitors, TaqMan and PCR primers used in the in vitro functional assays depicted in Figs. 1-4, 6-7. Suppl. Table 3 provides a phenotypic summary of the in vitro assays in Figs. 2-4. Suppl. Fig. 1 shows characterization of exosomes secreted from PC3-derived cell lines profiled in Fig. 1C. Suppl. Figs. 2-4 confirms miR-888 and miR-891a misexpression using miRNA mimics, miRNA inhibitors, and lentiviral miRNA mimic reagents in human prostate cells analyzed in Figs. 2-7. Suppl. Fig. 5 represents predicted miR-888 and miR-891a binding sites found in the 3'UTR regions of RBL1, SMAD4, KLF5, and TIMP2 transcripts validated in Fig. 6A using luciferase reporter assays. Suppl. Fig. 6 indicates that miR-888 targets RBL1, SMAD4, KLF5, and TIMP2 possess cell type specific expression in LNCaP when compared to PC3-N cells in Fig. 6B-C. Suppl. Fig. 7 shows functional evidence that miR-888 targets RBL1 and TIMP2 to control proliferation and invasion activities assayed in Figs. 2-3. Suppl. Fig. 8 implicates that miR-888 and miR-891a act synergistically to regulate prostate cell proliferation depicted in Fig. 2 and provides a model of how the miR-888 cluster regulates prostate cancer progression.</p>