Supplemental manganese improves the relative growth, net assimilation and photosynthetic rates of salt‐stressed barley

Abstract

Previous results in our laboratory indicated that a reduced Mn concentration in the leaves of barley was highly correlated with the reduced relative growth and net assimilation rates of salt‐stressed plants. If Mn deficiency limits the growth of salt‐stressed barley, then increasing leaf Mn concentrations should increase growth. In the present study, the effect of supplemental Mn on the growth of salt‐stressed barley (Hordeum vulgare L. cv. CM 72) was tested to determine if a salinity‐induced Mn deficiency was limiting growth. Plants were salinized with 125 mol m−3 NaCl and 9.6 mol m−3 CaCl2. Supplemental Mn was applied in 2 ways: 1) by increasing the Mn concentration in the solution culture and 2) by spraying Mn solutions directly onto the leaves. Growth was markedly inhibited at this salinity level. Dry matter production was increased 100% in salt‐stressed plants treated with supplemental Mn to about 32% of the level of nonsalinized controls. The optimum solution culture concentration was 2.0 mmol m−3, and the optimum concentration applied to the leaves was 5.0 mol m−3. Supplemental Mn did not affect the growth of control plants. Further experiments showed that supplemental Mn increased Mn concentrations and uptake to the shoot. Supplemental Mn increased the relative growth rate of salt‐stressed plants and this increase was attributed to an increase in the net assimilation rate; there were no significant effects on the leaf area ratio. Supplemental Mn also increased the net photosynthetic rate of salt‐stressed plants. The data support the hypothesis that salinity induced a Mn deficiency in the shoot, which partially reduced photosynthetic rates and growth.