Supplemental Figures 1-5 and Supplemental methods from Definition of Smad3 Phosphorylation Events That Affect Malignant and Metastatic Behaviors in Breast Cancer Cells

Abstract

<p>Supplemental Figures 1-5 and Supplemental Methods. Supplementary Figure 1. Blockade of Smad3 phosphorylation at the C-terminal and linker region by infection with adenoviral Smad3 mutants. Supplementary Figure 2. Mutation of the Smad3 linker phosphorylation sites enhances the TGF-β1-induced growth arrest and apoptosis. Supplementary Figure 3. Smad3 linker phosphorylation suppresses Smad3 transcriptional activity. Supplementary Figure 4. Mutation of Smad3 linker phosphorylation sites promotes the TGF-β-induced EMT and invasive activity. Supplementary Figure 5. Mutation of the Smad3 linker phosphorylation sites significantly inhibits the expression of embryonic transcription factors, mammosphere formation, and colony formation on soft agar.</p>