Supplemental Figures 1-14 from Depleting Tumor-NQO1 Potentiates Anoikis and Inhibits Growth of NSCLC

Abstract

<p>Supplemental Figure 1: Tumor-NQO1 overexpression leads to poor prognosis in lung cancer patients. Supplemental Figure 2: shNQO1-B knockdown of NQO1 leads to decreased growth in soft agar. Supplemental Figure 3: shNQO1-B knockdown of NQO1 in H292 cells leads to decreased growth in soft agar. Supplemental Figure 4: Dicoumarol inhibits growth of A549 cells in soft agar. Supplemental Figure 5: Mac220 inhibits growth of A549 cells in soft agar. Supplemental Figure 6: Stable overexpression of NQO1 in H596 (NQO1 null) lung cancer cells causes increased growth in soft agar. Supplemental Figure 7: Transient knockdown of NQO1 in H596 LPC-NQO1 cells causes decreased growth in soft agar. Supplemental Figure 8: shNQO1-B knockdown of NQO1 in A549 cells decreases cell invasion. Supplemental Figure 9: Stable overexpression of NQO1 in H596 NQO1 null cells causes increased invasion. Supplementary Figure 10: Transient knockdown of NQO1 using siRNA inhibits invasion of HCC1171 cells. Supplemental Figure 11: Stable knockdown of NQO1 in A549 cells causes loss of PARP- 1 protein expression. Supplemental Figure 12: Knockdown of NQO1 reduces ALDH (high) activity in Pancreas cancer cells. Supplemental Figure 13: Knockdown of NQO1 reduces ALDH (high) activity in Prostate cancer cells. Supplemental Figure 14: NQO1 knockdown does not inhibit short-term viability our long-term survival of non-transformed human bronchial epithelial cells.</p>