Supplemental Data from ARRB1-Promoted NOTCH1 Degradation Is Suppressed by OncomiR miR-223 in T-cell Acute Lymphoblastic Leukemia

Abstract

<p>Supplemental methods. Supplementary Figure 1. ARRB1 inhibits the cell proliferation and tumor progression of human T-ALL cells. Supplementary Figure 2. RNA-Seq expression profile of T-ALL patients and the aberrant expression of ARRB1in T-ALL samples and cell lines. Supplementary Figure 3. ARRB1 promotes the degradation and ubiquitination of ICN1 in Molt4 cells. Supplementary Figure 4. The co-immunoprecipitation (Co-IP) results of ARRB1 interaction with E3 ubiquitin ligases of Notch1 in T-ALL cells. Supplementary Figure 5. DTX1 is essential for the inhibitory effect of ARRB1 in T-ALL cells. Supplementary Figure 6. ARRB1-derived miR-223 sponge BUTR effectively induces apoptosis in CCRF-CEM T-ALL cells.</p>