Abstract
We study the effect of annular pupil filter which was inserted in one of the two excitation beams in two-color excitation fluorescence microscopy. By our numerical experiments, we note that the reinforced side lobes induced by annular aperture is evidently suppressed and at the same time the large-area photodetector used in two photons imaging can be used. The axial elongate along the optical axis is also decreased. However, one price that must be paid for these is inefficient use of the light source.
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