Abstract
ABSTRACTMethods of super-resolving light microscopy (SRM) have found an exponentially growing range of applications in cell biology, including nuclear structure analyses. Recent developments have proven that Single Molecule Localization Microscopy (SMLM), a type of SRM, is particularly useful for enhanced spatial analysis of the cell nucleus due to its highest resolving capability combined with very specific fluorescent labeling. In this commentary we offer a brief review of the latest methodological development in the field of SMLM of chromatin designated DNA Structure Fluctuation Assisted Binding Activated Localization Microscopy (abbreviated as fBALM) as well as its potential future applications in biology and medicine.
Highlights
The chromatin distribution within the cell nucleus can be visualized through highlighting the DNA
In our latest publication [30] we presented a novel single molecule-based method that advances DNABALM [23] to imaging the chromatin within the cell nucleus and enables studying nuclear architecture at an unprecedented level of structural resolution
This methodology termed as DNA structure fluctuation assisted Binding Activated Localization Microscopy (BALM) relies on a moderate nuclear membrane permeabilization followed by the delivery of a fluorescent DNA-binding dye inside the fixed cell nucleus
Summary
The chromatin distribution within the cell nucleus can be visualized through highlighting the DNA. Isolated DNA of λ-phage was imaged with a resolution below 10 nm using Binding Activated Localization Microscopy (BALM) [23].
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