Abstract
The new technique of spin trapping has been applied to a biological system for the first time. The light induced generation of O 2 − by chloroplasts in the presence of oxygen has been shown by the production of the O 2 − adduct of the spin trap 5,5-dimethyl-1-pyrroline-1-oxide. The O 2 − adduct was detected by electron spin resonance spectroscopy. Methyl viologen enhanced the production of the O 2 − adduct thus providing support for the hypothes is that methyl viologen accepts electrons from the primary acceptor of photosystem I and subsequently reduces O 2 to O 2 −.
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