Supernatants of Bifidobacterium longum and Lactobacillus plantarum Strains Exhibited Antioxidative Effects on A7R5 Cells.

Yusheng Wang,Jianxin Zhao,Hao Zhang,Wei Chen,Wenwei Lu,Shumao Cui,Zhifeng Fang,Qixiao Zhai

doi:10.3390/microorganisms9020452

Yusheng Wang, Jianxin Zhao + Show 6 more

Open Access

https://doi.org/10.3390/microorganisms9020452

Copy DOI

Abstract

Vascular reactive oxygen species (ROS) play an essential role in cardiovascular diseases and the antioxidative effects of probiotics have been widely reported. To screen the probiotic strains that may prevent cardiovascular diseases, we tested the antioxidative effects of supernatants of different Bifidobacterium and Lactobacillus strains on A7R5 cells. Preincubation with supernatants of B. longum CCFM752, L. plantarum CCFM1149, or L. plantarum CCFM10 significantly suppressed the angiotensin II-induced increases in ROS levels and increased catalase (CAT) activity in A7R5, whereas CCFM752 inhibited NADPH oxidase activation and CCFM1149 enhanced the intracellular superoxide dismutase (SOD) activity simultaneously. Treatment with CCFM752, CCFM1149, or CCFM10 supernatants had no significant impact on transcriptional levels of Cat, Sod1, Sod2, Nox1, p22phox, or p47phox, but altered the overall transcriptomic profile and the expression of genes relevant to protein biosynthesis, and up-regulated the 60S ribosomal protein L7a (Rpl7a). A positive correlation between Rpl7a expression and intracellular CAT activity implied that Rpl7a may participate in CAT synthesis in A7R5. Supernatant of CCFM752 could also down-regulate the expression of NADPH oxidase activator 1 (Noxa1) and angiotensinogen in A7R5. Collectively, the probiotic strains CCFM752, CCFM1149, and CCFM10 exhibited antioxidative attributes on A7R5 cells and might help to reduce the risk of cardiovascular diseases.

Highlights

Reactive oxygen species (ROS) in blood vessel walls comprise an important risk factor for cardiovascular diseases such as hypertension [1,2] and atherosclerosis [2,3]
CAT activity, while CCFM752 suppressed NADPH oxidase activation and CCFM1149 increased intracellular superoxide dismutase (SOD) activity in A7R5 cells, all of which contributed to an increased intracellular antioxidative capacity
The increased intracellular CAT and SOD levels after incubation with the supernatants were probably not associated with a transcriptional increase in Cat, Sod1, or Sod2, and the inhibition of NADPH oxidase might not be associated with transcriptional changes in Nox1, p22phox, and p47phox

Summary

Introduction

Reactive oxygen species (ROS) in blood vessel walls comprise an important risk factor for cardiovascular diseases such as hypertension [1,2] and atherosclerosis [2,3]. They function as signaling molecules in vascular walls, and are involved in basic physiological processes such as angiogenesis [4] and vasoconstriction [5]. ROS might promote the proliferation and extracellular matrix secretion of vascular smooth muscle cells (VSMC) and result in vascular hypertrophy [7] and fibrosis [8].

Objectives

Methods

Results

Discussion

Conclusion