Abstract
Hydroxysafflor yellow A (HSYA) is the main bioactive ingredient of XBJ injection. At first, the stability of HSYA in solution and in a Xuebijing injection was investigated, then the mechanisms of the increased stability of HSYA in the XBJ injection were investigated to provide useful information on clinical safety. HSYA stability was investigated as a function of pH and temperature in aqueous solution and an XBJ injection, following the guidelines from the International Conference on Harmonisation of Technical Requirements for Registration of Pharmaceuticals for Human Use. Products were identified by UPLC-MS/MS. HSYA reaction followed first-order kinetics under all conditions. The half-life of HSYA in XBJ was almost 40 times longer than in aqueous solution. The activation energies of HSYA reaction in aqueous solution and XBJ were calculated to be 78.53 and 92.90 kJ∙mol−1 by using Arrhenius equation. The results indicated that HSYA was more stable in XBJ than in aqueous solution. Two products were identified and the mechanism was intra-molecular nucleophilic substitution. The excellent stability of HSYA in XBJ injection partly due to the micelles formed in the injection. The study may provide clues for compatibility in TCM prescription and also provide useful information for further preparation technology research of HSYA and assessment of clinical safety of XBJ.
Highlights
Hydroxysafflor yellow A (HSYA) is a pharmacologically active chalcone found in Flos Carthami, a medicinal and edible herb from Eurasia
Under weak to moderate with previous findings [27], we found that the flavanone-chalcone isomerization equilibrium was alkaline conditions, the hydroxyl group at C-20 is ionized firstly, followed by intra-molecular regulated by alkalinity of the buffer
HSYA and the other with previous findings [27], we found that the flavanone-chalcone isomerization equilibrium was four active ingredients (PF, DSS, ferulic acid degradation (FA) and Sen I) of XBJ were detected in EEX while dextrose was regulated byWSX
Summary
Hydroxysafflor yellow A (HSYA) is a pharmacologically active chalcone found in Flos Carthami, a medicinal and edible herb from Eurasia. Stability of active ingredients of herbal drugs is one of important factors affecting the quality, safety and efficacy of herbal drugs [12]. HSYA may have thermo and stability anddegrade may degrade best of there are few reports on the chemical stability of in aqueous solution or in XBJ. As an important our knowledge, there are few reports on the chemical stability of HSYA in aqueous solution or in TCM injection, the therapeutic efficacythe andtherapeutic safety of XBJ haveand caused extensive concern. Theabove parameters associated with the degradation of HSYA infor aqueous solution and XBJ under condition were estimated. The effects of WSX and EEX into and four bioactive ingredients of XBJ (PF), injection was separated on an SPE column water-soluble. HSYA degradation pathways and the factors underlying its influenced stability in XBJ were proposed
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
