Superior and Novel Carbohydrate Antigen 125 Electrochemical Sensor Based on 4-(2-(Naphthalen-1-Yl)benzo[b]thiophen-3-Yl)benzaldehyde

Abstract

Ovarian cancer (OC) is a cancer type that observed as very widespread among women around the world, and that a high mortality rate. Cancer antigen-125 (CA-125) is a tumor marker widely used for OC. CA-125 antigen could be released both against OC by the body and by OC tumors, and OC can be researched by following the CA-125 antigen level in the blood medium. It is available immunoassay tests to detect CA-125 on the market. However, incompatibility can be seen in these tests in some cases. Therefore, there is a need for immunosensors that can detect CA-125 antigens with higher sensitivity and a faster. Herein, we produced an electrochemical sensorwith 4-(2-(naphthalen-1-yl)benzo[b]thiophen-3-yl)benzaldehyde-based (NBTB) that be a benzothiophene derivative to detect CA-125 more rapidly and safely by electrochemical techniques. Measurements are performed on produced immonusensor with CV, EIS, and DPV techniques to research properties of immonusensor like interference effects, scan rate, incubation time, LOD and LOQ values, and CA-125 concentration. Electrochemical sensorhas wide range of linear region as 0.01-10 ng/ml and 50-1000 ng/ml. LOD, and LOQ were found as 0.022468 ng/ml and 0.067405 ng/ml at (S/N=3), respectively. Interference and artificial serum results reveal that this electrode is a promising electrode for CA125 antigen determination for the OC. As a result, NBTB antibody is a novel and unique antibody could be promising antibody for CA125 antigen detection.