Abstract
In Argentina, many Flavivirus were recognised including West Nile virus (WNV). During 2009 several strains of Culex Flavivirus (CxFV), an insect-specific flavivirus, were isolated in the same region where circulation of WNV was detected. Hence, the objective of this study was to analyse the effect of co-infection in vitro assays using CxFV and WNV Argentinean strains in order to evaluate if CxFV could affect WNV replication. Our results showed that WNV replication was suppressed when multiplicity of infection (MOI) for CxFV was 10 or 100 times higher than WNV. Nevertheless, in vivo assays are necessary in order to evaluate the superinfection exclusion potential.
Highlights
The genus Flavivirus comprises over 70 viruses that include several human pathogens such as Yellow fever, Dengue virus (DENV1-4), Zika virus (ZIKV) and West Nile viruses (WNV)
Members of this genus are enveloped, positive-sense singlestranded RNA viruses. Their genome is approximately 11 kb in length, which includes a short 5 ́non-coding region (5 ́NCR), a single long open-reading frame encoding the structural proteins capsid (C), premembrane envelope (E), seven non-structural proteins (NS1, NS2a, NS2b-NS3-NS4a-NS4b-NS5) and a 3 ́non-coding region (3 ́NCR). Based on their host associations, flaviviruses have been grouped into insect-specific flaviviruses (ISFV) which cause a persistent infection in the insect, and vertebrate viruses with either no known vector.[1] in the last decade novel insect-specific viruses, which are host-restricted to replication in invertebrate cells, were discovered in mosquitoes
In Argentina, several medically important mosquito-borne flaviviruses (MBFV) have been detected in recent years.[9,10,11,12] Since 1997, Argentina has experienced the re-emergence of DENV1-4, which represented a growing public health problem
Summary
The genus Flavivirus (family Flaviviridae) comprises over 70 viruses that include several human pathogens such as Yellow fever, Dengue virus (DENV1-4), Zika virus (ZIKV) and West Nile viruses (WNV). We performed co-infection in vitro assays, in order to evaluate the impact on WNV replication in co-infected cultures cells with different multiplicity of infection (MOI) for CxFV. In order to assess the in vitro potential of CxFV Otero2009 infection on WNV-Eq001 replication in the mosquito cell line, triplicate cultures of Aedes albopictus C6/36 cells in T25-cm2 flasks were inoculated.
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