Superficial Bifurcated Microflow Phantom for High-Frequency Ultrasound Applications

Abstract

To evaluate and optimize high-frequency ultrasound (HFUS) imaging techniques that visualize the morphology of microscale vasculatures, many studies have used flow phantoms with straight channels. However, the previous phantoms lack the complexity of microvessels to simulate a realistic vascular environment in a shallow depth. This study was aimed at devising a new protocol for fabrication of a microflow phantom with bifurcated geometry at a superficial region. The proposed protocol involved the following features: (i) a bifurcated flow tract model 300 µm in diameter was debossed on the surface of a tissue slab made of polyvinyl alcohol cryogel, and (ii) a wall-less lumen was created via bonding tissue slabs to put a lid on the debossed flow tract. The structure of the created microflow phantom was evaluated using 2-D and 3-D power Doppler imaging with a 30 MHz HFUS modality. Ultrasound imaging revealed that the desired flow tract with bifurcation was successfully created in the phantom at a depth of 2-5 mm from the ultrasound probe. The diameters of the flow tract measured in the axial direction were 307 ± 3.7 µm in the parent branch and 232 ± 18.2 and 256 ± 23.3 µm in the two daughter branches, respectively. The experiments revealed that the proposed protocol for creating a microscale intricate flow tract with desired dimensions and depth is valid. This new phantom will facilitate further improvement in the ultrasound technologies for the precise visualization of superficial complex vasculatures such as those in skin layers.