Abstract
The goal of this work is isolation and characterization of biological active compounds in fish caviar, viscera and fillet obtained from fresh water carp (Cyprinus carpio L.) from Tikveš Lake, Republic of Macedonia. A solid-liquid extraction by Soxhlet method was applied in order to obtain total extracts from analyzed samples of animal origin. During the experimental investigation, the influence of some process parameters over the total yield of extract was examined. The SFE-CO2 was introduced as a desirable alternative in the defined system solvent-lyophilized matrixes. Further, the influence of the operating parameters: operating temperature, operating pressure and extraction time on the yield of total extract, were determined through maximization of the functional dependency. Obtained total extracts were characterized by GC-FID method for qualitative and quantitative determination of the presence of fatty acids. From resulting chromatograms it was concluded that these total extracts contain monounsaturated (MUFA) and polyunsaturated (PUFA) fatty acids.
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