Supercritical fluid extraction and clean-up of organochlorine pesticides and polychlorinated biphenyls in mussels

Abstract

The simultaneous extraction and clean-up of mussel samples followed by gas chromatography with electron-capture detection and mass spectrometric confirmation of 15 organochlorine pesticides (OCPs) and 11 polychlorinated biphenyls (PCBs) is developed using Florisil sorbent in the supercritical fluid extraction cell. The method detection limits vary from 1 to 10 ng/g for OCPs and from 2 to 15 ng/g for PCBs. Mean reproducibilities of 11% and 10% and mean recoveries of 80% and 53%, respectively, for OCPs and PCBs are obtained. The feasibility of the proposed supercritical fluid extraction method was confirmed by analyzing a certified reference material and mussels collected from Taiwan region. The method is simple, rapid and requires only small amounts of samples and solvents. It may serve as a screening protocol for the determination of OCPs and PCBs in mussels on a routine basis.