Supercritical Fluid Extract of Putranjiva roxburghii Wall. Seeds Mitigates Fertility Impairment in a Zebrafish Model

Acharya Balkrishna,Anurag Varshney,Lakshmipathi Khandrika,Pradeep Nain,Monali Joshi

doi:10.3390/molecules26041020

Acharya Balkrishna, Anurag Varshney + Show 3 more

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https://doi.org/10.3390/molecules26041020

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Journal: Molecules	Publication Date: Feb 15, 2021
Citations: 4	License type: CC BY 4.0

Affiliation: Patanjali Research Foundation

Abstract

Putrajeevak (Putranjiva roxburghii Wall.; synonym Drypetes roxburghii (Wall.) Hurus) seeds have been used since ancient times in the treatment of infertility in the Ayurvedic system of medicine in India. In this study, the oil component of Putrajeevak seeds (PJSO) was extracted using the supercritical fluid extraction (SCFE) method using liquid CO2 and the constituents were analyzed using gas chromatography-flame ionized detectorand high-performance thin-layer chromatography. PJSO contained trace amounts of β-sitosterol with oleic and linoleic acids as the major fatty acid constituents. Male and female zebrafish were mutagenized with N-ethyl-N-nitrosourea (ENU) and fish that produced less than 20 viable embryos were selected for the study. SCFE oil extracts from the P. roxburghii seeds were used in this study to reverse fertility impairment. The mutant fish were fed with PJSO for a period of 14 days and the rates of fertility, conception, and fecundity were determined with wild-type healthy fish as a breeding partner. Treatment with PJSO increased the ovarian follicle count as well as the number of mature eggs, while reducing the number of ovarian cysts. Sperm count as well as sperm motility were greatly enhanced in the ENU-mutagenized male zebrafish when treated with PJSO. The results obtained in this study demonstrate the effectiveness of P. roxburghii seed oil in reversing impaired fertility in both male and female zebrafish models.

Highlights

Putrajeevak (Putranjiva roxburghii Wall.; synonym Drypetes roxburghii (Wall.) Hurus) seeds have been used in the traditional Indian system of Ayurveda since ancient times for the treatment of infertility in both males and females
The batch of oil used for these studies was obtained from powdered seeds (2 kg) of Putrajeevak (Putranjiva roxburghii Wall.) using a supercritical CO2 extraction procedure to obtain 60 gm of the oil extract (PJSO) with a yield of 3%
The mixture from the Putrajeevak seed oil (PJSO) was compared with a standard mixture (37 fatty acid methyl esters (FAME)) using Gas chromatography-flame ionized detection (GC-FID)

Summary

Introduction

Putrajeevak (Putranjiva roxburghii Wall.; synonym Drypetes roxburghii (Wall.) Hurus) seeds have been used in the traditional Indian system of Ayurveda since ancient times for the treatment of infertility in both males and females. Many classical medicinal texts such as Candra-Nighan. T.u(16th Century A.D.), and others have an extensive discussion on the use of P. roxburghii in alleviating infertility. The plant belongs to the Euphorbiaceae family and is native to Southeast Asia, the Indian subcontinent, Japan, southern China, and New Guinea. Its antipyretic and anti-inflammatory properties are thought to be useful in fertility and gynecological ailments [2]. Several different phytochemicals have been identified from P. roxburghii root, leaf, and stem including glycosides, saponins, triterpenes, and flavonoids

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