Abstract
On-line bioassay-directed fractionation of environmental samples is important because of the need to assign toxicological activity to certain classes of chemicals. Supercritical fluid chromatography (SFC) was used to fractionate a coal tar standard (SRM 1597) and these fractions were successfully directed into the Salmonella microsuspension mutagenicity assay. The reverse-mutation assay used in these experiments measures mutagenicity in Salmonella typhimurium TA 98 and was performed in a total volume of approximately 200 μl. Coupling of SFC with the microsuspension assay yielded quantitative recovery of the coal tar mutagenic activity, and the SFC chromatogram was compared to the mutagenicity profile to determine the activity of each fraction. This method of coupling SFC with bioassays may have broad applicability for fractionating various environmental samples and for identifying a wide range of biological activities in such samples.
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