Supercritical Carbon Dioxide Extraction Process for Hibiscus taiwanensis S. Y. Hu and Tyrosinase Inhibitory Activity of Its Extract

Abstract

In this study, the bioactive components in the stem of Hibiscus taiwanensis S. Y. Hu were extracted by supercritical carbon dioxide (SC-CO2), and the inhibition activity of the extract tyrosinase was analyzed. When the particle size of the powder was controlled to be 50 mesh, the effects of temperatures (40, 45, 50, 55, and 60 °C), pressures (15, 20, 25, 30, and 35 MPa), concentrations of entrainment agent (ethanol) (60, 80, and 95%) and CO2 flow rates (80, 100, 120, and 140 L/min) on the extraction rate, respectively, were studied with the single-variable method. The optimal parameters included an extraction temperature of 55 °C, an extraction pressure of 30 MPa, 80% ethanol as the entrainment agent, and the CO2 flow rate of 100 L/min. Under the optimal conditions, the extraction rate of Hibiscus taiwanensis S. Y. Hu reached more than 0.5% within 2 h. At the same time, the chemical compositions of the extract were investigated by using high-resolution liquid chromatography-mass spectrometry (LC-MS) equipped with a linear ion trap-electrostatic field orbital trap. In addition, the related activity of extract in the stem Hibiscus taiwanensis S. Y. Hu was evaluated by tyrosinase inhibition experiments. The results showed that the inhibition rate of the extract with a concentration of 0.25 mg/mL to tyrosinase reached 35.60%, indicating that the extract by SC-CO2 had the potential to be used for whitening active raw materials.