Abstract
The xanthones from mangosteen ( Garcinia mangostana) pericarp was extracted with and without alcohol as entrainer at 30 MPa and 50 °C in supercritical carbon dioxide (SC-CO 2). It was found that entrainer increases the rate of extraction and yield substantially. SC-CO 2 + ethanol (4%) at 20 MPa, 40 °C was investigated by HPLC/LC–ESI-MS for characterization of the xanthones. From liquid chromatography electron spray ionization mass spectrometry (LC–ESI-MS) spectra, 12 components were structurally correlated and confirmed based on the fragmentation characteristics and information available in the literature. The occurrence of these xanthone in SC-CO 2 extract is reported for first time in G. mangostana species. The antioxidant activities of SC-CO 2 were investigated by 2,2-diphenyl-1-picrylhydrazyl (DPPH) radicals (IC 50 = 41.8 μg/mL), lipid peroxidation (IC 50 = 21.5 μg/mL), hydroxyl radicals site specific (IC 50 = 30.0 μg/mL) and hydroxyl radicals non-site specific (IC 50 = 23.1 μg/mL). From cytochrome c assay it was observed that SC-CO 2 extract can reduce ferric cytochrome c to ferrocytochrome c. The results showed that SC-CO 2 had great potential as antioxidant agent.
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