Supercritical Antisolvent-based Technology for Preparation of Vitamin D 3 Proliposome and Its Characteristics

Abstract

Vitamin D 3 (VD 3) proliposomes (VDP), consisted of hydrogenated phosphatidycholine (HPC) and VD 3, were prepared using supercritical anti-solvent technology (SAS). The effects of operation conditions (temperature, pressure and components) on the VD 3 loading in VDP were studied. At the optimum conditions of pressure of 8.0 MPa, temperature of 45°C, and the mass ratio of 15.0% between VD 3 and HPC, the VD 3 loading reached 12.89%. VD 3 liposomes (VDL) were obtained by hydrating VDP and the entrapment efficiency of VD 3 in VDL reached 98.5%. The morphology and structure of VDP and VDL were characterized by SEM (scanning electron microscope), TEM (transmission electron microscope) and XRD (X-ray diffractometer). The structure of VD 3 nanoparticles in HPC matrix was formed. The size of VDL with an average diameter of about 1μm was determined by dynamic light scattering instrument (DLS). The results indicated that VDP can be made by SAS and VDL with high entrapment efficiency can be formed easily via the hydration of VDP.