Supercharged Mechanical Stromal-cell Transfer (MEST).

Abstract

Summary:PRP and fat-derived stromal-cell applications are the 2 most commonly used methods in regenerative medicine. PRP has a wide spectrum of indications. Mechanical methods have become very popular recently in fat-derived stromal-cell applications due to the advantages they provide. Combining these 2 methods has produced more successful results. To date, this combination has been in the form of combining 2 products obtained separately just before they are administered to the patient. In this study, fat tissue and blood samples obtained from eight volunteers were mixed with PPP as a new idea not previously reported in the literature, and stromal cells were obtained mechanically with sharp blades (adinizing). Later, the obtained PRP was added to the final product and became “supercharged.” The results were tested by the dual fluoroscopy method for cell number and viability, and the results obtained were analyzed statistically. By adding the plasma to the oil before stromal cells were obtained and cutting with sharp blades by mechanical separation, twice the volume and 4.7 times more cells were obtained compared with that obtained in the saline group (P < 0.001). We believe that the reason for this is the “binding” effect of the proteins in the plasma. This approach provided a higher cell count by using PPP, which is a “waste product,” and in addition, the potential efficiency was increased by adding PRP. However, the clinical results of this innovative method should be evaluated with advanced clinical studies.