Abstract
AbstractMicroRNA (miRNA) expression profiles hold promise as biomarkers for diagnostics and prognosis of complex diseases. Herein, we report a super‐resolution fluorescence imaging‐based digital profiling method for specific, sensitive, and multiplexed detection of miRNAs. In particular, we applied the DNA‐PAINT (point accumulation for imaging in nanoscale topography) method to implement a super‐resolution geometric barcoding scheme for multiplexed single‐molecule miRNA capture and digital counting. Using synthetic DNA nanostructures as a programmable miRNA capture nano‐array, we demonstrated high‐specificity (single nucleotide mismatch discrimination), multiplexed (8‐plex, 2 panels), and sensitive measurements on synthetic miRNA samples, as well as applied one 8‐plex panel to measure endogenous miRNAs levels in total RNA extract from HeLa cells.
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