Super-Ovulated Chinese Sika Deer (Cervus Nippon) and Assessment of Embryo Transfer at Developmental Stages

Abstract

The Embryo transfer has been commercially recognized reproductive technique in the world. The study was conducted at a Jiutai local breeding deer farm in China. The breeding protocol was developed for embryo transfer in sika deer to be implemented at farm levels and under wild conditions. Success rates of the technique was measured through indicators like number of follicles developed as a result of a specific treatment protocol, super ovulation rates, and quality of embryos. The male sika deer (Cervus Nippon) were then selected in terms of semen quality, libido, and sperm productivity. Embryo recovery was performed by surgical technique, of which the percentage of the embryo developmental stages was measured, in terms of Blastocyst, Morula, Non-Fertilized. The Hinds (sample size=12) were synchronically induced by inserting a controlled internal drug release (CIDR)devices, injected with Follicle stimulating Hormone (FSH) i.m for 2-4days .The fertilization for hinds was achieved by natural mating after 4days after CIDR devices withdrawal .Embryo recovery was performed on Day 12(n=6) and Day13(n=6)of the breeding season. The major objective of this research was to evaluate the super- stimulation protocols and determine the frequency of recovered transferable embryo in sika deer. Nevertheless, there were no significant differences P<0.05 between the numbers of corpus luteum, Follicles, Blastocysts and Morula from both uterine horns. The embryo recovery showed positive outcomes of blastocyst, morula for sustainable genetic banking of pure sika deer.