Sundarban Honey Confers Protection against Isoproterenol-Induced Myocardial Infarction in Wistar Rats.

Rizwana Afroz,E M Tanvir,Md Ibrahim Khalil,Nadia Alam,Md Sabir Hossain,Nurul Karim,Siew Hua Gan

doi:10.1155/2016/6437641

Abstract

The present study was designed to investigate the cardioprotective effects of Sundarban honey (SH) in rats with isoproterenol- (ISO-) induced myocardial infarction. Adult male Wistar Albino rats were pretreated with Sundarban honey (5 g/kg) daily for a period of 6 weeks. After the treatment period, ISO (85 mg/kg) was subcutaneously injected into the rats at 24 h intervals for 2 days. ISO-induced myocardial damage was indicated by increased serum cardiac specific troponin I levels and cardiac marker enzyme activities including creatine kinase-MB, lactate dehydrogenase, aspartate transaminase, and alanine transaminase. Significant increases in serum total cholesterol, triglycerides, and low-density lipoprotein-cholesterol levels were also observed, along with a reduction in the serum high-density lipoprotein-cholesterol level. In addition to these diagnostic markers, the levels of lipid peroxide products were significantly increased. The activities of antioxidant enzymes such as superoxide dismutase, glutathione peroxidase, and glutathione reductase were significantly decreased in the hearts after ISO-induced myocardial infarction. However, pretreatment of ischemic rats with Sundarban honey brought the biochemical parameters to near normalcy, indicating the protective effect of Sundarban honey against ISO-induced ischemia in rats. Histopathological findings of the heart tissues further confirmed the biochemical findings, indicating that Sundarban honey confers protection against ISO-induced oxidative stress in the myocardium.

Highlights

Sundarban honey (SH) a wild multifloral honey produced by Apis dorsata bees is one of the most renowned types of honey from Bangladesh
There were no significant differences in the body weights observed between the groups (Table 1)
In rats pretreated with SH prior to ISO treatment, the heart weights were significantly reduced compared with rats treated with ISO alone

Summary

Introduction

Sundarban honey (SH) a wild multifloral honey produced by Apis dorsata bees is one of the most renowned types of honey from Bangladesh. Apis dorsata bees collect the nectar from Sundarban plant species, for example, Khalsi [Aegiceras corniculatum (L.) Blanco], Kakra [Bruguiera gymnorrhiza (L.) Lam], Keora [Sonneratia apetala (B.) Ham], and Goran [Ceriops decandra (G.) Ding Hou] [1]. Investigation on pharmacological benefits of SH revealed that it is one of the most renowned types of honey from Bangladesh and conferred protection against oxidative stress-induced liver and kidney damage [2, 3]. Free radicals and reactive oxygen species have been implicated in many diseases and have a deleterious effect on cardiac function.

Methods

Results

Conclusion