SUN-151 OFFSPRING OF OBESE MICE DEMONSTRATE MINOR CHANGES IN KIDNEY

Abstract

Recently it was shown that acquired paternal obesity may result in obesity, metabolic syndrome, insulin resistance and increased risk of the development of diabetes in the offspring. Some studies have shown the possibility of changes in inner organs of the offspring. Based on this we ran a study of body composition, metabolic changes and kidney status in offspring from male mice with obesity induced with diet. Male C57Bl6/N mice were placed for 12 weeks on either a high fat diet (HFD, 45% kcal fat) or a low fat diet (LFD, 10% kcal fat) and then mated with females on LFD. All parents after mating and all offspring were on regular mice chow. Offspring were tested by nuclear magnetic resonance for body composition. Glucose tolerance test (GTT) and insulin sensitivity test (IST) were performed. Kidney status was studied. HFD offspring (HFDO) and LFD offspring (LFDO) were tested at the age of 20 days, 6 weeks, 6 and 12 months. There were small but significant differences in body weight between diet groups offspring in both males and females as early as 20 days and in males at 1.5 and 12 months; in all cases HFDO body weights were greater than LFDO. The higher body weight was the result of mostly higher fat content accumulated in subcutaneous, retroperitoneal and mesenteric depots, but not in gonadal and interscapular (brown adipose tissue). In HFDO animals GTT and IST changes were observed at 12 months and the increase of insulin secretion was the highest at 6 weeks. Kidney weight increased in all groups with age and was consistently greater in males than in females. However, when adjusted for body weight, kidney weight went down with increasing age and was higher at 1.5 months in both HFDO males and females than LFDO. HFD with 45% of fat for 12 weeks results in obesity and changes in body composition in male mice that epigenetically effects the offspring. Male offspring demonstrate more metabolic changes than females. Kidney weight increase were observed in HFDO at 1.5 months timepoint.