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Abstract
SUMO (Small ubiquitin‐like modifier), is a member of the family of ubiquitin‐like polypeptides that become covalently attached to intracellular target proteins. SUMOylation can alter the function, location, and/or half‐life of target proteins. Here we describe three SUMO proteins and three SUMO‐like proteins in Chlamydomonas reinhardtii. The three SUMO genes show differential RNA expression depending on environmental conditions. Western analysis also shows a shift in proteins recognized by the Chlamydomonas reinhardtii SUMO antibodies in response to changes in environmental conditions. The three SUMO‐like proteins are novel fusion proteins with C‐terminal SUMO. Chlamydomonas reinhardtii subcellular fractionation reveals SUMO proteins are conjugated to proteins involved in various cellular processes. Proposed SUMOylated target proteins can be resolved by one‐dimensional and two‐dimensional western analysis.Supported by NSF and the Nebraska Research Council.
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