Abstract
Vibrational information recorded by infrared-visible sum frequency generation spectroscopy was used to study the adsorption of a derivated vitamin (biocytin) on different substrates and its subsequent reaction with a protein (avidin). No reaction is observed on metallic substrates. When the experiments are carried out with a CaF 2 substrate in the total internal reflection configuration, significant changes of the CH and NH vibrations can be related to the specific bonding of avidin to biocytin.
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