Abstract
A METHOD for the estimation of sulphydryl groups in hair and other insoluble proteins by means of the specific sulphydryl reagent, 1-(4-chloromercuriphenylazo)-naphthol-2, has recently been reported by Scott and Flesch1. In this method, the hair is pulverized, treated with water at 100° or an aqueous solution of a swelling agent at 40° and shaken with a solution of the reagent in amyl acetate, the decrease in concentration of the reagent in the amyl acetate layer being measured colorimetrically.
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