Sulphur nutrition of sunflower ( Helianthus annuus) as affected by nitrogen supply: Effects on vegetative growth, the development of yield components, and seed yield and quality

Abstract

The effects of sulphur and nitrogen nutrition on the growth and yield components of a hybrid confectionery sunflower (cv. Kernel) were investigated in a glasshouse experiment using controlled S and N supplies that ranged from deficient to more than adequate, and in which the S supplies of some plants were changed at defined stages of growth. S deficiency delayed floret initiation and anthesis but not seed maturity; N deficiency delayed all developmental phases including seed maturity. S and N deficiencies reduced plant height and leaf area. N deficiency caused a reduction in leaf number, but S deficiency did not. Both S and N deficiencies reduced yield by reducing the number of seeds per plant and by decreasing single seed weight. Oil concentration in seeds was the same for all levels of S supplied, but it was reduced by increased N supply. Changing the S supply at the end of floret initiation and at anthesis indicated that floret number and therefore seed number per plant were mainly established by the S suplly before the end of floret initiation. Single seed weight responded positively to increased S supply after floret initiation but only if the N supply was adequate; there was no response to increased S supply after anthesis. Concentrations of S and N in plant organs increased with increasing S and Nsupplies, but seeds were much less responsive than vegetative organs. Plants grown on high N but low S had the highest concentrations of N in their tissues, but the highest S concentrations did not occur in high S low N plants. Cysteine and methionine decreased by 30% in seeds of S-deficient but N-sufficient plants, whereas arginine increased by 34% compared with nutrient-adequate plants. We suggest that an adequate supply of S to young sunflower plants is required, particularly up to floret initiation, to obtain large floret numbers and maximum leaf area. An adequate supply of S is also required between the end of floret initiation and anthesis to prevent floret abortion and allow development of large seeds. Sulphur stress in sunflower during seed filling results in kernels with low levels of essential S-containing amino acids.