Abstract

AbstractThe purpose of this study was to evaluate the toxic effect of sulphide on thermophilic methanogenic bacteria, pre‐cultivated in UASB‐reactors fed with and without sulphate, at 55°C and different pH levels. For granular sludge, precultivated in the presence of sulphate, the inhibition caused by hydrogen sulphide was dependent on the pH imposed. At alkaline pH the inhibitory effect of hydrogen sulphide was higher than at neutral or acidic pH. The effect of sulphide could not be described in terms of free hydrogen sulphide level only. For dispersed sludge, pre‐cultivated in the absence of sulphate, the inhibition caused by free hydrogen sulphide was independent of the pH, and the toxic effect of sulphide could be described in terms of free hydrogen sulphide.

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