Abstract
The specificity of some histochemical methods such as Alcian blue (at different pH and electrolyte levels), toluidine blue (at various pH levels) and ferric thiocyanate ion complex was tested on standard material possessing sulphate, phosphate or carboxyl groups. The best method of distinguishing between these three groups was Alcian blue in the presence of various concentrations of magnesium chloride. Similar tests were conducted on human duct saliva and the results compared with those obtained on the standard material. Strongly ionized groups, reacting positively with Alcian blue at pH 0.6, Alcian blue in a solution of 1.0 M MgCl 2 and with toluidine blue at pH 1.09 were found in saliva from sublingual glands. The comparison of the results from standard and salivary material suggests that the strongly ionized groups are sulphate.
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