Abstract
Soluble enzyme preparations from bovine ovary, corpus luteum and adrenal cortex and from rat testis sulfurylated estradiol-17β in the presence of ATP and Mg ++. Two major products were tentatively identified by chromatographic comparisons as estradiol-3-sulfate and estrone sulfate 2. In addition, two unidentified, highly-polar derivatives were formed from estradiol-17β in relatively small amounts by ovary, corpus luteum and testis. Estradiol-17-sulfate, which was formed readily in similar extracts of rat liver, was not formed in detectable amounts by the extracts of ovary, corpus luteum, testis, and adrenal cortex under the conditions described.
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