Abstract
The studies concerned the expression of sulfurtransferases and cystathionine beta-synthase in six human leukemia cell lines: B cell acute lymphoblastic leukemia-B-ALL (REH cells), T cell acute lymphoblastic leukemia-T-ALL (DND-41 and MOLT-4 cells), acute myeloid leukemia—AML (MV4-11 and MOLM-14 cells), and chronic myeloid leukemia—CML (K562 cells). Reverse transcription-polymerase chain reaction (RT-PCR) and Western blot analysis were performed to determine the expression of thiosulfate sulfurtransferase, 3-mercaptopyruvate sulfurtransferase, gamma-cystathionase, and cystathionine beta-synthase on the mRNA and protein level. Interestingly, we found significant differences in the mRNA and protein levels of sulfurtransferases and cystathionine beta-synthase in the studied leukemia cells. The obtained results may contribute to elucidating the significance of the differences between the studied cells in the field of sulfur compound metabolism and finding new promising ways to inhibit the proliferation of various types of leukemic cells by modulating the activity of sulfurtransferases, cystathionine beta-synthase, and, consequently, the change of intracellular level of sulfane sulfur as well as H2S and reactive oxygen species production.
Highlights
Leukemia is a type of cancer that affects the blood and bone marrow
The studies were performed in human leukemia cell lines: REH (B cell acute lymphoblastic leukemia, B-cell acute lymphocytic leukemia (B-ALL)); DND-41 and MOLT-4 (T cell acute lymphoblastic leukemia, T-cell acute lymphocytic leukemia (T-ALL)); MOLM-14 and MV4-11; and K562
We examined the expression of three sulfurtransferases and cystathionine beta-synthase in different types of human leukemia cell lines
Summary
Leukemia is a type of cancer that affects the blood and bone marrow. Leukemia is classified by the cell phenotype (lymphocytic leukemia vs. myelogenous leukemia) and the rapidity of the clinical course (acute-fast-growing vs. chronic-slow-growing) (Figure 1).There are four major types of leukemia: acute lymphoblastic leukemia (ALL), acute myeloid leukemia (AML), chronic myeloid leukemia (CML), and chronic lymphocytic leukemia (CLL) [1,2].Acute leukemias are malignant clonal disorders of the hematopoietic system. Leukemia is a type of cancer that affects the blood and bone marrow. Leukemia is classified by the cell phenotype (lymphocytic leukemia vs myelogenous leukemia) and the rapidity of the clinical course (acute-fast-growing vs chronic-slow-growing) (Figure 1). There are four major types of leukemia: acute lymphoblastic leukemia (ALL), acute myeloid leukemia (AML), chronic myeloid leukemia (CML), and chronic lymphocytic leukemia (CLL) [1,2]. Acute leukemias are malignant clonal disorders of the hematopoietic system. Acute leukemias are marked by the diffuse replacement of bone marrow with abnormal immature and undifferentiated hematopoietic cells, resulting in reduced numbers of erythrocytes and platelets in the peripheral blood [3]. Acute lymphoblastic leukemia—ALL—can be categorized as B-cell acute lymphocytic leukemia (B-ALL) and T-cell acute lymphocytic leukemia (T-ALL) [4]
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