Sulfur-Mediated Polycarbonate Polyurethane for Potential Application of Blood-Contacting Materials.

Peichuang Li,Wanhao Cai,Yuancong Zhao,Jin Wang,Xin Li,Hong Zhang

doi:10.3389/fbioe.2022.874419

Abstract

In this study, a sulfur-mediated polycarbonate polyurethane (PCU-SS) is developed by mimicking the catalyzing ability of glutathione peroxidase (GPx) on nitric oxide (NO) in the human body. The PCU-SS is endowed with the capability to produce NO based on disulfide bonds, which could strongly improve the biocompatibility of the materials. The characterization results indicate that PCU-SS could not only decrease the adhesion of platelets but also enhance the capability of anti-thrombus. Moreover, it is shown that PCU-SS has a good compatibility with endothelial cells (ECs), while has a marked inhibition capacity of the proliferation of smooth muscle cells (SMCs) and macrophages (MA). Meanwhile, the result of animal implantation experiments further demonstrates the good abilities of PCU-SS on anti-inflammation, anti-thrombus, and anti-hyperplasia. Our results offer a novel strategy for the modification of blood-contacting materials based on disulfide bonds. It is expected that the PCU-SS could shed new light on biocompatibility improvement of cardiovascular stents.

Highlights

Since the first performance of percutaneous coronary angioplasty in 1977 (Grüntzig, 1978), the direct intervention of interventional therapy has been widely used in improving the living quality of patients
Similar results are observed in the fluorescence microscope images where adherent platelets are stained with rhodamine (Figure 4D), as well as in the statistical results (Figure 4E). All these results indicate that PCU-SS with GSNO can effectively inhibit platelet adhesion, aggregation, and activation
The glutathione peroxidase (GPx)-like function of PCU-SS coating could induce nitric oxide (NO) release in the blood environment, improving the biocompatibility of the modified samples

Summary

INTRODUCTION

Since the first performance of percutaneous coronary angioplasty in 1977 (Grüntzig, 1978), the direct intervention of interventional therapy has been widely used in improving the living quality of patients. One important technique is tissue engineering including cell seeding (Zhu et al, 2008; Raina et al, 2014), which could be applied to vascular stent surfaces to achieve surface endothelialization This method can achieve the closest state of the normal tissue, the endothelial tissue is restricted by cumbersome procedures and the significant risk of tissue shedding during implantation. The modification of the structure and function of materials can be optimized by means of physical blending or chemical synthesis Physical means such as the drug loading (Roopmani et al, 2019; Wang et al, 2021) or functional component doping (Xu et al, 2019) can significantly improve the biocompatibility of vascular interventional implants. Our strategy based on disulfide bonds opens up a new avenue for the preparation of new blood-contacting materials

MATERIALS AND METHODS

RESULTS AND DISCUSSION

ETHICS STATEMENT

CONCLUSION