Sulfur-based Mixotrophic Vanadium (V) Bio-reduction towards Lower Organic Requirement and Sulfate Accumulation

Abstract

Although remediation of toxic vanadium (V) [V(V)] pollution can be achieved through either heterotrophic or sulfur-based autotrophic microbial reduction, these processes would require a large amount of organic carbons or generate excessive sulfate. This study reported that by using mixotrophic V(V) bio-reduction with acetate and elemental sulfur [S(0)] as joint electron donors, V(V) removal performance was enhanced due to cooccurrence of heterotrophic and autotrophic activities. Deposited vanadium (IV) was identified as the main reduction product by scanning electron microscopy, energy-dispersive X-ray spectroscopy, X-ray diffraction and X-ray photoelectron spectroscopy. Based on 16S rRNA gene amplicon sequencing, qPCR and genus-specific reverse transcription qPCR, it was observed that V(V) was likely detoxified by heterotrophic V(V) reducers (e.g., Syntrophobacter, Spirochaeta and Geobacter). Cytochrome c, intracellular nicotinamide adenine dinucleotide and extracellular polymeric substances were involved in V(V) reduction and binding. Organic metabolites synthesized by autotrophs (e.g., Thioclava) with energy from S(0) oxidation might compensate electron donors for heterotrophic V(V) and sulfate reducers. Less sulfate was accumulated presumably due to activities of sulfur-respiring genera (e.g., Desulfurella). This study demonstrates mixotrophic microbial V(V) reduction can save organic dosage and avoid excessive sulfate accumulation, which will be beneficial to bioremediation of V(V) contamination.