Abstract
Glycosaminoglycans (GAGs) are an integral part of the extracellular matrix and glycocalix, i.e. the closest cellular environment. They are abundant in –OH groups and their bioactivity is also associated with the presence of negatively charged –SO3H functionalities. Therefore, we have investigated and discussed the influence of these functional units on mesenchymal stem cells (MSCs) behaviour using single component and mixed self-assembled monolayers of alkanethiols with –SO3H and –OH end groups. In the absence of serum, MSCs attachment, spreading, cytoskeleton organisation and motility were significantly influenced by the surface chemistry. We found that the sulfonic groups induce star-like cell shape with very intense actin staining and a high density net of filopodia that enlarge from the base of lamellipodia structures. Moreover, this response is concentration dependent and is apparent only for very short culture time in the presence of serum.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
