Sulfonamide and tetracycline resistance genes in total- and culturable-bacterial assemblages in South African aquatic environments.

Satoru Suzuki,Mitsuko Ogo,Brent Newman,Hideshige Takada,Tatsuya Koike

doi:10.3389/fmicb.2015.00796

Abstract

Antibiotic resistant bacteria are ubiquitous in the natural environment. The introduction of eﬄuent derived antibiotic resistance genes (ARGs) into aquatic environments is of concern in the spreading of genetic risk. This study showed the prevalence of sulfonamide and tetracycline resistance genes, sul1, sul2, sul3, and tet(M), in the total bacterial assemblage and colony forming bacterial assemblage in river and estuarine water and sewage treatment plants (STP) in South Africa. There was no correlation between antibiotic concentrations and ARGs, suggesting the targeted ARGs are spread in a wide area without connection to selection pressure. Among sul genes, sul1 and sul2 were major genes in the total (over 10-2 copies/16S) and colony forming bacteria assemblages (∼10-1 copies/16S). In urban waters, the sul3 gene was mostly not detectable in total and culturable assemblages, suggesting sul3 is not abundant. tet(M) was found in natural assemblages with 10-3 copies/16S level in STP, but was not detected in colony forming bacteria, suggesting the non-culturable (yet-to-be cultured) bacterial community in urban surface waters and STP eﬄuent possess the tet(M) gene. Sulfamethoxazole (SMX) resistant (SMXr) and oxytetracycline (OTC) resistant (OTCr) bacterial communities in urban waters possessed not only sul1 and sul2 but also sul3 and tet(M) genes. These genes are widely distributed in SMXr and OTCr bacteria. In conclusion, urban river and estuarine water and STP eﬄuent in the Durban area were highly contaminated with ARGs, and the yet-to-be cultured bacterial community may act as a non-visible ARG reservoir in certain situations.

Highlights

Antibiotic resistance genes (ARGs) are found in the clinical and the natural environment, which can eventually produce antibiotic resistant bacteria (ARB)
In recent monitoring in the Philippines we showed that the total bacterial community in seawater possessed minor sulfonamide resistance gene sul3, which was not detected in colony forming bacteria (Suzuki et al, 2013)
Drug Contamination The distribution of antibiotic concentrations in surface waters and sewage treatment plants (STP) effluent showed that SMX was a major contaminant along with trimethoprim, which is a combination drug

Summary

Introduction

Antibiotic resistance genes (ARGs) are found in the clinical and the natural environment, which can eventually produce antibiotic resistant bacteria (ARB). Antibiotics and ARB are released to the environment from hospitals, livestock facilities, and sewage treatment plants (STP) (Pruden et al, 2013). Antibiotics are decomposed and diluted in the aquatic environment water, even at low concentrations they may act as signaling molecules in microbes (Fajardo and Martinez, 2008). Selection of ARG mutation by very low concentrations of antibiotics. ARGs in South Africa is reported (Gullberg et al, 2011). It is, critical to understand the fate of released antibiotics, ARB and ARGs in the environment, and whether residual ARGs in the environment pose a risk to humans.

Objectives

Methods

Results