Abstract
Supernatant fractions obtained by high speed centrifugation of rat kidney and liver homogenates incorporated sulfate- 35S into an endogenous lipid. The radioactive product was identified as cholesterol sulfate- 35S by thin-layer chromatography and by co-crystallization with authentic cholesterol sulfate. More sulfate- 35S was incorporated into the lipid fraction when non-radioactive cholesterol was added to the incubation mixture. When tritium labeled cholesterol was added to the supernatant fraction from kidney, tritium labeled cholesterol sulfate was formed. Following parenteral administration of sulfate- 35S, cholesterol sulfate- 35S was identified as the predominant radioactive lipid in the kidney and liver. These studies demonstrate the presence of cholesterol sulfotransferase activity in rat kidney and liver.
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