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Abstract
The aim of the present study was to investigate the gastroprotective activity of a sulfated-polysaccharide (PLS) fraction extracted from the marine red algae Gracilaria caudata and the mechanism underlying the gastroprotective activity. Male Swiss mice were treated with PLS (3, 10, 30 and 90 mg·kg−1, p.o.), and after 30 min, they were administered 50% ethanol (0.5 mL/25 g−1, p.o.). One hour later, gastric damage was measured using a planimeter. Samples of the stomach tissue were also obtained for histopathological assessment and for assays of glutathione (GSH) and malondialdehyde (MDA). Other groups were pretreated with l-NAME (10 mg·kg−1, i.p.), dl-propargylglycine (PAG, 50 mg·kg−1, p.o.) or glibenclamide (5 mg·kg−1, i.p.). After 1 h, PLS (30 mg·kg−1, p.o.) was administered. After 30 min, ethanol 50% was administered (0.5 mL/25g−1, p.o.), followed by sacrifice after 60 min. PLS prevented-ethanol-induced macroscopic and microscopic gastric injury in a dose-dependent manner. However, treatment with l-NAME or glibenclamide reversed this gastroprotective effect. Administration of propargylglycine did not influence the effect of PLS. Our results suggest that PLS has a protective effect against ethanol-induced gastric damage in mice via activation of the NO/KATP pathway.
Highlights
Marine organisms are sources of numerous new compounds with multiple pharmacological properties [1]
The mechanisms and pathophysiology underlying the effects of ethanol on the organs of the digestive tract are not yet completely understood
We confirmed that ethanol induced gastrophathy (61.3 ± 18.9 mm2) and that PLS
Summary
Marine organisms are sources of numerous new compounds with multiple pharmacological properties [1]. May present many pharmacological opportunities [5,6,7,8] In algae, these sulfated-polysaccharides are complex macromolecular constituents of the extracellular matrix, and they play an important role in the mechanical, osmotic, and ionic regulation of these beings [9,10]. These sulfated-polysaccharides are complex macromolecular constituents of the extracellular matrix, and they play an important role in the mechanical, osmotic, and ionic regulation of these beings [9,10] Investigation of these biomolecules has been steadily increasing in recent years owing to their broad potential development as antithrombotic, anti-viral, anticoagulant, antioxidant, anti-inflammatory, and anti-proliferative agents [11,12,13,14]. Studies of sulfated-polysaccharide extracted from sea algae (hereafter referred to as “PLS” throughout this manuscript) in ethanol-induced gastric damage models are scarce
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