Sulfated glycosaminoglycans synthesized by fibroblast, smooth muscle and endothelium-like cells grown in culture

Abstract

Monolayer cultures of fibroblast, smooth muscle and endothelium-like cells incorporated 35SO 4 2− into glycosaminoglycans of the extracellular, pericellular and intracellular compartments. These glycosaminoglycans have been identified on the basis of electrophoretic mobility, enzymatic degradation with specific mucopolysaccharidases and by the type of degradation products formed. The sulfated glycosaminoglycans from the extracellular pool of the three cell types show a similar composition, while the intracellular and pericellular pools of the three cells have a different glycosaminoglycans composition. They differ in the relative proportion of heparitin sulfate and chondroitin sulfate and in the structure of isomeric chondroitin sulfate.