Abstract
Normal human bile was collected under aseptic condition. Subsequently, it was frozen without preservative until use. A mixture of 40 bile samples was fractionated centrifugally. in the presence of salts. The resulting fractions showed strong coagulating effects on calcium carbonate suspension in water. The major fractions contained sulfated glycoproteins as the principal component and small amounts of acidic glycosaminoglycans. The present data together with previous observations suggest that sulfated glycoproteins and acidic glycosaminoglycans detected in the present normal bile samples might be a result of bacterial infection.
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