Abstract
Sulfate fluxes and sulfate metabolites in Entamoeba histolytica were characterized employing [ 35S]sulfate as a marker. Sulfate was taken up both across the plasma membrane and by pinocytosis; in growth medium (sulfate concentration, 1,1 mM) total uptake was 1.5 μmol h −1 (5 × 10 7 cells) −1. The fate of sulfate within the cells was investigated by thin-layer chromatography. Major metabolites (together >3 μmol (5 × 10 7 cells) −1) were monoethyl sulfate and 3-cholesteryl sulfate; both those products were released into the growth medium. As minor components we identified the activated sulfate derivatives, adenosine-5′-phosphosulfate and 3′-phosphoadenosine-5′-phosphosulfate. In addition, up to 10% of the sulfate taken up was incorporated into high-molecular weight material (possibly proteoglycans). We propose that sulfurylation of cholesterol may play a role in controlling membrane sterol content.
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