Sulawesi propolis induces higher apoptotic activity and lower inflammatory activity in a rat endometriosis model.

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Endometriosis has a major impact on women's quality of life. The two primary pathologies are chronic inflammation and altered apoptotic activity. Sulawesi propolis has been shown to have known anti-inflammatory and pro-apoptotic properties in other diseases. To investigate the effects of Sulawesi propolis in the rat endometriosis model. An autologous endometriosis model was created in 60 female Wistar rats by laparotomy. Rats were divided into four groups (n=15 in each group): control group (CG), dienogest group (DG), propolis 50mg/kg body weight (BW)/day (P50) group, and propolis 100mg/kg BW/day (P100) group. Each treatment group was divided into three different treatment durations (n=5 in each treatment group): 2, 4 and 6 weeks. After treatment, laparotomy was performed to determine endometriotic tissue growth, apoptosis [caspase-3 and Bcl-2-associated X/Bcl-2 (Bax/Bcl)] and inflammation [prostaglandin-E2 (PGE2) and interleukin-1B (IL-1B)]. A significant difference was seen in endometriotic tissue growth between the P50 group and the CG, with the greatest reduction in the P50 6-week (P50-6) group, reaching 70.66% of the initial area. Highest Bax/Bcl-2 mRNA expression was shown in the P50-4 and P100-4 groups, highest caspase-3 expression was shown in the P50-2 and P50-4 groups, and lowest IL-1B expression was shown in the P50-4 group; all differed significantly from the CG. No significant difference in PGE2S mRNA was found between the groups. Sulawesi propolis extract suppressed endometriotic tissue growth in the rat model by increasing apoptotic activity. The effects were time-dependent, with 50mg/kg BW as the optimal dose.