Abstract
ABSTRACTRetrotransposons, a subclass of mobile genetic elements, have been discovered in many organisms. In this study, we identified a plant-specific retrotransposon (Sukkula) in the human genome (24 different DNA samples) by using retrotransposon-based molecular marker technique: inter-retrotransposon amplified polymorphism. There were five different groups related to the ages of the subjects. The polymorphism ratios were 8%–100% among all samples, 10%–91% among females (12 subjects) and 13%–100% among males (12 subjects). Moreover, we also observed 8%–91% polymorphism ratios when comparing males to females. To the best of our knowledge, this is one of the first reports on plant-specific retrotransposons in the human genome. The obtained findings are expected to contribute to broadening the knowledge about plant retrotransposons in the human genome and their role in human genome evolution.
Highlights
Transposable elements (TEs) are DNA sequences that can move within or, occasionally, between genomes
One such marker is IRAP, which is based on polymerase chain reaction (PCR) amplification using primers designed to amplify in an outward direction from the conserved LTR sequences
IRAP-PCR was performed with the Sukkula-specific primer (30 GGAACGTCG GCATCGGGCTG5') [15]
Summary
Transposable elements (TEs) are DNA sequences that can move within or, occasionally, between genomes. Mobile genetic elements; human genome; IRAP; polymorphism different organs within the same organism, especially in plants In these investigations, many molecular marker techniques based on retrotransposon movements have been used to detect mobility in genomes [10]. Many molecular marker techniques based on retrotransposon movements have been used to detect mobility in genomes [10] One such marker is IRAP (inter-retrotransposon amplified polymorphism), which is based on polymerase chain reaction (PCR) amplification using primers designed to amplify in an outward direction from the conserved LTR (long terminal repeat) sequences. This way, the genomic distance between two LTRs is amplified [11]. Sukkula elements are non-autonomous retroelements, named large retrotransposon derivative (LARD) elements [14]
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