Abstract
The unusual responsiveness of the thyroid tissue of goldfish to small amounts of hypophyseal thyrotropin has provided in these experiments the basis for a sensitive, inexpensive assay procedure. In a series of tests in which 115 two-inch-long fish were used, marked growth of the epithelium of the thyroid follicles was produced by crude and relatively purified preparations from the pituitaries of mammals, birds, amphibia, and fishes. One Parkes-Rowlands guinea pig unit, 0.25 mg of a purified, standardized mammalian pituitary thyrotropic powder was much larger than the minimum dose producing thyroid stimulation in the goldfish.Groups of 5 fish receiving the same treatment were kept in one-gallon jars. The hormone was administered intraperitoneally in 5 successive daily injections of 0.2 cc each day, dissolved or suspended in the desired dilution of Ringer's solution. At autopsy, performed on the sixth day, the lower jaw was removed and trimmed. This piece of tissue, about 2 mm by 3 mm in size, was fixed in ...
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