Abstract
Abstract. Litter size in sheep can be improved by introgression of the FecB gene. Genetic markers closely linked to the FecB locus can be used to detect gene carriers. The efficiency of introgression can be increased by using marker assisted selection. Individuals of the prolific breeds Tyrolean Mountain sheep (n = 41), Romanov (n = 43), East Friesian Milksheep (n = 35) and Merinoland sheep (n = 265) were genotyped with the microsatellite markers OarAElOl and BMI 329 linked to the FecB gene to estimate allele frequencies of both microsatellites in these breeds. The frequencies of the microsatellite alleles linked to the FecB gene in Merinoland sheep were 0.07 and 0.1 respectively. The alleles occured also in the other breeds (Tyrolean Mountain sheep, Romanov and East Friesian Milksheep) but with mostly higher frequencies. Furthermore the segregation of the microsatellite alleles was studied in families produced by mating Merinoland sheep ewes (Fec+/Fec+) with two heterozygous Booroola Merino rams (Fec+/FecB) in order to use these markers within an indirect gene test. The recombination rate between BM 1329 and OarAElOl was 13 cM (LOD score 14.6).
Highlights
Litter size in sheep has a low heritability coefficient and a large variability between and within breeds (ELSEN et al, 1994)
WE1MANN et al.: Suitability of microsatellites BMI329 and OarAElOl as markers into different sheep breeds with effects on Ovulation rate and litter size, a single autosomal segregation was discovered in the Booroola strain (DAVIS et al, 1982)
The aim of this study was to determine the allele frequencies of microsatellites BM1329 and OarAElOl in four sheep breeds in order to test the suitability of these two microsatellites as markers for the introgression of the FecB locus into the Merinoland sheep
Summary
Litter size in sheep has a low heritability coefficient and a large variability between and within breeds (ELSEN et al, 1994). WE1MANN et al.: Suitability of microsatellites BMI329 and OarAElOl as markers into different sheep breeds with effects on Ovulation rate and litter size, a single autosomal segregation was discovered in the Booroola strain (DAVIS et al, 1982). Linkage to Fee was first demonstrated with two microsatellite markers (OarAElOl and OarHH55) and epidermal growth factor (EGF) from human chromosome 4 (MONTGOMERY et al, 1993). The aim of this study was to determine the allele frequencies of microsatellites BM1329 and OarAElOl in four sheep breeds in order to test the suitability of these two microsatellites as markers for the introgression of the FecB locus into the Merinoland sheep. Furtheron the segregation of microsatellites alleles BM1329 and OarAElOl should be demonstrated in families of German Merinoland sheep in order to improve litter size and the recombination rate between BM1329 and OarAElOl was calculated
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