SU‐GG‐I‐149: Positive MRI Contrast Agent Based on Gd2O3 Nanoparticles for Cell Tracking

Abstract

Purpose: In this study nanoparticles of gadolinium oxide have been investigated for intracellular uptake and capacity to generate a positive contrast. For this purpose two novel composed of a) silicon‐based nanocomposite polymer(NCP), b) Diethylene glycol (DEG) based coating material, both loaded with gadolinium(III) oxide (Gd2O3) nanoparticles were used. Method and Materials: This study would be involved with nanoparticles composed gadolinium (III) oxide, a) emulsified with a silicon‐based nanocomposite polymer and b) capped with Diethylene glycol (DEG) by polyol method starting from of Gd2O3 large particles, leading to Gd2O3 nano size capping with polymers. Prpoerties evaluation of nanoparticles were assessed previously by this group. Presently, cytotoxicity of gadolinium oxide nanoparticles in U‐87 MG cancer cells was evaluated. Furthermore relaxivity of particles in U‐87 cells were compared to particles in cell culture medium. Results: The results showed that Gd2O3‐labeled cells have shorter T1 and T2 relaxation times compared with untreated cells. The slope of r1 relaxivity (1/T1) vs. concentration curve in cell samples and ccm of Gd2O3 (NCP)were 10.87 and 10.60 s−1mM−1 and for Gd2O3 (deg) were 13.99, 13.66 s−1mM−1 respectively. The slope of r2 relaxivity (1/T2) vs. concentration curve in cell samples and ccm of Gd2O3 (NCP) were 17.26 and 15.13s−1mM−1 and for Gd2O3 (deg) were 16.35, 14.62 s−1mM−1 respectively. Conclision: A prominent difference in signal intensity was observed, indicating that Gd2O3 nanoparticles can be used as a positive contrast agent for cell labeling. No appreciable toxicity was observed with a Gd2O3 nanoparticles. Conclusion: Gadolinium‐Nanocomposite Polymer Emulsion is well characterised and potentialy useful positive contrast agent for magnetic resonance molecular imaging.