Abstract
1. 1. The entry of sugar into beef erythrocytes was found to be facilitated by a saturable, passive process with unusually high affinity for d-glucose and d-3-O- methylglucose , the Michaelis constants for these two sugars being 0.09 and 0.17 mM respectively. In other mammalian cells, the transport K m is about the same for these two sugars, and ranges between 6 and 15 mM. The transport V was about 0.1–0.2 μmole/min per ml for both sugars, slow by comparison to most other mammalian cells. 2. 2. The beef erythrocyte differed from the human cell but resembled the rabbit cell in that extracellular methyl glucose did not affect efflux, and intracellular methylglucose appeared not to affect influx. However, the inhibitory effects of glucose on methylglucose entry were stronger if the glucose was added some time prior to the addition of methylglucose. Since intracellular methyl glucose did not inhibit influx, the time dependent inhibition by glucose was likely due to glucose metabolism rather than intracellular glucose per se. Other kinetic criteria affirmed this conclusion.
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