Sugar Pills? Investigating Humphreys’ Homeopathic Specifics: Utilizing Liquid Chromatography-Tandem Mass Spectrometry for an In-Depth Look at Nineteenth-Century American Homeopathy

Abstract

Frederick K. Humphreys (1816-1900), founder of Humphreys Medicine Company, appeared at the rise of both greater acceptance and popularity of the homeopathy in the United States, as opposed to more orthodox medicine. Although bolstered by the relatively high cost and low success rate of traditional medicines, homeopathic remedies soon became a source of controversy due to the largely unknown ingredient lists and contradictory claims of effectiveness. Tandem mass spectrometry analysis (MS/MS) was used to pinpoint the ingredients of four “specifics” in Humphreys’ Homeopathic Remedies. Generated spectra showed similar base peak ions for each pill, leading to the hypothesis that each remedy contained the same basic ingredients. Further MS/MS analyses identified these base peak ions as adducts of sucrose (m/z 365, 381), its related ions (m/z 433, 203, 185), and apigenin (m/z 271). When placed into a historical context, the application of chemical analyses to study these early remedies can provide insight into the question of validity that evoked arguments during the late nineteenth century.